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| Durand, Jean-pierre; Goudard, Françoise; Pieri, Jacques; Escoubas, Jean-michel; Schreiber, Nathalie; Cadoret, Jean-paul. |
| Ferritin has been shown as being the principal iron storage in the majority of living organisms. In marine species, ferritin is also involved in high-level accumulation of Po-210. As part of our work on the investigation of these radionuclides' concentration in natural environment, ferritin was searched at the gene and protein level. Ferritin was purified from the visceral mass of the oyster Crassostrea gigas by ion-exchange chromatography and HPLC. SDS-PAGE revealed one band of 20 kDa. An Expressed Sequence Tag (EST) library was screened and led to the identification of two complementary DNA (cDNA) involved in ferritin subunit expression. The complete coding sequences and the untranslated regions (UTRs) of the two genes were obtained and a 5' Rapid... |
| Tipo: Text |
Palavras-chave: Iron; IRE; EST; Oyster. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-693.pdf |
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| Carrier, Gregory; Baroukh, Caroline; Rouxel, Catherine; Duboscq-bidot, Laetitia; Schreiber, Nathalie; Bougaran, Gael. |
| Tisochrysis lutea is a microalga species currently used in aquaculture as a feed for shellfish, oysters and shrimps. It also has many other potential industrial applications, such as the production of neutral lipids for biofuels or the production of ω-3 fatty acids for nutraceuticals (human food complements). To efficiently exploit the potential of this microalga, however, higher lipid productivities are needed. To this end, improvement programs need to be developed and optimized. The diversity of strains available in microalgae has not yet been exploited in such improvement programs. In this study, the intra-strain diversity was observed and exploited to increase neutral lipid productivity. New clonal strains with higher neutral lipid productivity were... |
| Tipo: Text |
Palavras-chave: Microalgae; Genome; Improvement program; Lipids; Tisochrysis lutea. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00411/52204/52927.pdf |
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| Coulombier, Noémie; Nicolau, Elodie; Le Déan, Loic; Barthelemy, Vanille; Schreiber, Nathalie; Brun, Pierre; Lebouvier, Nicolas; Jauffrais, Thierry. |
| Nephroselmis sp. was previously identified as a species of interest for its antioxidant properties owing to its high carotenoid content. In addition, nitrogen availability can impact biomass and specific metabolites’ production of microalgae. To optimize parameters of antioxidant production, Nephroselmis sp. was cultivated in batch and continuous culture conditions in stirred closed photobioreactors under different nitrogen conditions (N-repletion, N-limitation, and N-starvation). The aim was to determine the influence of nitrogen availability on the peroxyl radical scavenging activity (oxygen radical absorbance capacity (ORAC) assay) and carotenoid content of Nephroselmis sp. Pigment analysis revealed a specific and unusual photosynthetic system with... |
| Tipo: Text |
Palavras-chave: Lutein; Natural products; Nutrients; Peroxyl radical; Siphonaxanthin. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00645/75699/76604.pdf |
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| Le Chevanton, Myriam; Garnier, Matthieu; Lukomska, Ewa; Schreiber, Nathalie; Cadoret, Jean-paul; Saint-jean, Bruno; Bougaran, Gael. |
| Interactions between photosynthetic and non-photosynthetic microorganisms play an essential role in natural aquatic environments and the contribution of bacteria and microalgae to the nitrogen cycle can lead to both competitive and mutualistic relationships. Nitrogen is considered to be, with phosphorus and iron, one of the main limiting nutrients for primary production in the oceans and its availability experiences large temporal and geographical variations. For these reasons, it is important to understand how competitive and mutualistic interactions between photosynthetic and heterotrophic microorganisms are impacted by nitrogen limitation. In a previous study performed in batch cultures, the addition of a selected bacterial strain of Alteromonas sp.... |
| Tipo: Text |
Palavras-chave: Interactions; Nitrogen limitation; Microalgae; Bacteria; Chemostat; Dissolved organic matter (DOM); Dunaliella. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00344/45529/45087.pdf |
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| Gilliers, Camille; Le Pape, Olivier; Desaunay, Yves; Bergeron, Jean-pierre; Schreiber, Nathalie; Guerault, Daniel; Amara, Rachid. |
| Indicators of growth and condition were used to compare the habitat quality of nurseries of juvenile sole (Solea solea L.) in the Bay of Biscay, based on one survey in 2000. The four biological indicators are poorly correlated with each other, suggesting that no single measure may give an adequate description of fish health and of its habitat's quality. Growth indicators showed significant differences between northern and southern areas. Juveniles from the two southernmost nurseries, the Gironde estuary and the Pertuis Antioche, displayed significant lower otolith increment widths and mean sizes. These differences were inversely related to water temperature and unrelated to genetic or age differences, and are unlikely to be due to limiting trophic... |
| Tipo: Text |
Palavras-chave: Erika oil spill; RNA/DNA; Otolith daily increment width; Essential fish habitat. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-3963.pdf |
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| Charrier, Aurelie Georgette; Berard, Jean-baptiste; Bougaran, Gael; Carrier, Gregory; Lukomska, Ewa; Schreiber, Nathalie; Fournier, Flora; Charrier, Aurelie; Rouxel, Catherine; Garnier, Matthieu; Cadoret, Jean-paul; Saint-jean, Bruno. |
| Microalgae have a diversity of industrial applications such as feed, food ingredients, depuration processes and energy. However, microalgal production costs could be substantially improved by controlling nutrient intake. Accordingly, a better understanding of microalgal nitrogen metabolism is essential. Using in silico analysis from transcriptomic data concerning the microalgae Tisochrysis lutea, four genes encoding putative high-affinity nitrate/nitrite transporters (TlNrt2) were identified. Unlike most of the land plants and microalgae, cloning of genomic sequences and their alignment with complementary DNA (cDNA) sequences did not reveal the presence of introns in all TlNrt2 genes. The deduced TlNRT2 protein sequences showed similarities to NRT2... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00253/36467/35044.pdf |
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| Le Chevanton, Myriam; Garnier, Matthieu; Bougaran, Gael; Schreiber, Nathalie; Lukomska, Ewa; Berard, Jean-baptiste; Fouilland, E.; Bernard, Olivier; Cadoret, Jean-paul. |
| Previous studies have demonstrated that bacteria influence microalgal metabolism, suggesting that the selection and characterization of growth-promoting bacteria should offer a new strategy for improving industrial algal cultivation. In the present study, 48 cultivable bacteria were isolated from marine microalgae species and identified using 16S rRNA phylogenetic analysis. The recovered bacteria were found to be members of the α- and γ-Proteobacteria, Cytophaga–Flavobacterium–Bacteroides (CFB) and gram-positive monophyletic clusters. To address the effect of these bacteria on the growth of Dunaliella sp. individually, an experimental high-throughput tool was developed to simultaneously compare replicated associations. A two-step approach was used to... |
| Tipo: Text |
Palavras-chave: Microalgae; Dunaliella; Bacteria; Interaction; Bacterial diversity. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00142/25334/24768.pdf |
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| Garnier, Matthieu; Bougaran, Gael; Pavlovic, Marija; Berard, Jean-baptiste; Carrier, Gregory; Charrier, Aurelie; Le Grand, Fabienne; Lukomska, Ewa; Rouxel, Catherine; Schreiber, Nathalie; Cadoret, Jean-paul; Rogniaux, Helene; Saint-jean, Bruno. |
| Haptophytes are a diverse monophyletic group with a worldwide distribution, known to be significantly involved in global climate regulation in their role as a carbon sink. Because nitrogen is a major limiting macronutrient for phytoplankton in oceans and for cultures of microalgae, understanding the involvement of nitrogen availability in haptophyte carbon partitioning is of global and biotechnological importance. Here, we made an ecophysiological study coupled with comprehensive large scale proteomic analysis to examine differences of behavior in reaction to nitrogen availability changes between a wild type strain of Tisochrysis lutea (WTc1) and a mutant strain (2Xc1) known to accumulate more storage lipids. Strains were grown in chemostats and studied... |
| Tipo: Text |
Palavras-chave: Algae; Isochrysis; Lipids; Nitrogen; Reverse genomic: proteomic. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00356/46702/46904.pdf |
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